Coronaviruses Methods and Protocols
Book Title: Coronaviruses Methods and Protocols
Book Author: Helena Jane Maier, Erica Bickerton, Paul Britton
Book Format: EBook
Date published: 2015
Illustrator: Humana Press
ISBN: 978-1-4939-2437-0
Number Of Pages: 299
Descripción
Coronaviruses Methods and Protocols
In this book we aimed to describe a variety of techniques that refl ect the wide range of research currently being performed in the fi eld of coronavirology. However, most of the techniques described are also applicable to a wide variety of other virology fi elds, so we hope that this book will have wider appeal. As such, we have started this book with an overview chapter of current understanding of coronavirus replication and pathogenesis to introduce non specialist readers to the field.
Since the emergence of SARS-Coronavirus in 2003, numerous new coronaviruses have been identifi ed. The emergence of MERS-Coronavirus in 2012 and the continued occurrence of human cases highlight the importance of techniques to verify the presence of coronaviruses in a sample as well as identify new coronaviruses that may pose a potential threat to the health of both humans and livestock. As such, chapters have been chosen to describe identifi cation, diagnosis, and study of evolution of coronaviruses.
To allow the study of viruses, propagation and quantifi cation of virus is essential.
Therefore, we have included chapters describing preparation of cells and organ cultures useful in propagating coronaviruses and titration techniques. In addition, several techniques for analyzing virus function require purification of virus, so purification protocols suitable for different downstream techniques have been included.
The ability to reverse engineer virus genomes and recover recombinant viruses with defined mutations is invaluable in the progression of understanding the mechanisms for virus pathogenicity, viral protein and RNA function and understanding virus-host interactions.
Therefore, chapters describing two commonly used reverse genetics techniques for coronaviruses are included.
In this book we aimed to describe a variety of techniques that refl ect the wide range of research currently being performed in the fi eld of coronavirology. However, most of the techniques described are also applicable to a wide variety of other virology fi elds, so we hope that this book will have wider appeal. As such, we have started this book with an overview chapter of current understanding of coronavirus replication and pathogenesis to introduce non specialist readers to the field.
Since the emergence of SARS-Coronavirus in 2003, numerous new coronaviruses have been identifi ed. The emergence of MERS-Coronavirus in 2012 and the continued occurrence of human cases highlight the importance of techniques to verify the presence of coronaviruses in a sample as well as identify new coronaviruses that may pose a potential threat to the health of both humans and livestock. As such, chapters have been chosen to describe identifi cation, diagnosis, and study of evolution of coronaviruses.
To allow the study of viruses, propagation and quantifi cation of virus is essential.
Therefore, we have included chapters describing preparation of cells and organ cultures useful in propagating coronaviruses and titration techniques. In addition, several techniques for analyzing virus function require purification of virus, so purification protocols suitable for different downstream techniques have been included.
The ability to reverse engineer virus genomes and recover recombinant viruses with defined mutations is invaluable in the progression of understanding the mechanisms for virus pathogenicity, viral protein and RNA function and understanding virus-host interactions.
Therefore, chapters describing two commonly used reverse genetics techniques for coronaviruses are included.
CONTENIDO:
- 2. Identification of a Novel Coronavirus from Guinea Fowl Using Metagenomics
- 3. Serological Diagnosis of Feline Coronavirus Infection by Immunochromatographic Test
- 4. Estimation of Evolutionary Dynamics and Selection Pressure in Coronaviruses
- 5. The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration
- 6. The Preparation of Chicken Kidney Cell Cultures for Virus Propagation
- 7. Isolation and Propagation of Coronaviruses in Embryonated Eggs
- 8. Characterization of Human Coronaviruses on Well- Differentiated Human Airway Epithelial Cell Cultures
- 9. Quantification of Infectious Bronchitis Coronavirus by Titration In Vitro and In Ovo
- 10. Purification of Coronavirus Virions for Cryo-EM and Proteomic Analysis
- 11. Partial Purification of IBV and Subsequent Isolation of Viral RNA for Next-Generation Sequencing
- 12. Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses
- 13. Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
- 14. Protein Histochemistry Using Coronaviral Spike Proteins: Studying Binding Profiles and Sialic Acid Requirements for Attachment to Tissues
- 15. Identification of Protein Receptors for Coronaviruses by Mass Spectrometry
- 16. Single Particle Tracking Assay to Study Coronavirus Membrane Fusion
- 17. Studying Coronavirus–Host Protein Interactions
- 18. A Field-Proven Yeast Two-Hybrid Protocol Used to Identify Coronavirus–Host Protein–Protein Interactions
- 19. Investigation of the Functional Roles of Host Cell Proteins Involved in Coronavirus Infection Using Highly Specific and Scalable RNA Interference (RNAi) Approach
- 20. Transcriptome Analysis of Feline Infectious Peritonitis Virus Infection
- 21. Quantification of Interferon Signaling in Avian Cells
- 22. Studying the Dynamics of Coronavirus Replicative Structures
- 23. Preparation of Cultured Cells Using High-Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope
