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Molecular biotechnology: principles and applications of recombinant DNA 4th ed.

Titulo del libro: Molecular biotechnology: principles and applications of recombinant DNA

Book Description: Libro en inglés

Autor del libro: Bernard R. Glick, Jack J. Pasternak, Cheryl L. Patten

Edición de libro: 4th edition

Formato de libro: EBook

Date published: 2010

Illustrator: ASM Press

ISBN: 978-1-55581-498-4

Número de páginas: 1020

Libros de Medicina – Rincón Médico

Molecular biotechnology: principles and applications of recombinant DNA

Since the early 1970s, when recombinant DNA technology was first developed, there has been a veritable explosion of knowledge in the biological sciences. Since that time, with the advent of PCR, chemical DNA synthesis, DNA sequencing, monoclonal antibodies, directed mutagenesis, genomics, proteomics, and metabolomics, our understanding of and ability to manipulate the biological world have grown exponentially.
When the first edition of Molecular Biotechnology: Principles and Applications of Recombinant DNA was published in 1994, nearly all of the transgenic organisms that were produced included only a single introduced gene. Just 15 years later, it is not uncommon for researchers to engineer organisms by modifying both the activity and the regulation of existing genes while at the same time introducing entire new pathways. In 1994, only a handful of products produced by this new technology were available in the marketplace.
Today, molecular biotechnology has given us several hundred new therapeutic agents, with many more in the pipeline, as well as dozens of
transgenic plants. The use of DNA has become a cornerstone of modern forensics, paternity testing, and ancestry determination. Several new recombinant vaccines have been developed, with many more on the horizon. The list goes on and on. Molecular biotechnology really has lived up to its promise, to all of the original hype. It has been estimated that worldwide there are currently several thousand biotechnology companies employing tens of thousands of scientists. When the exciting science being done at universities, government labs, and research institutes around the world is factored in, the rate of change and of discovery in the biological sciences is astounding. This fourth edition of Molecular Biotechnology, building upon the fundamentals that were established in the previous three editions, endeavors to provide readers with a window on some of the major developments in this growing field in the past several years. Of necessity, we have had to be highly selective in the material that is included in this edition. Moreover, the window that we are looking through is moving. This notwithstanding, we both expect and look forward to the commercialization of many of these discoveries as well as to the development of new approaches, insights, and discoveries.

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  • Preface
  • Preface to the First Edition
chapter 1: The Development of Molecular Biotechnology
  • The Emergence of Molecular Biotechnology
  • Recombinant DNA Technology
  • Commercialization of Molecular Biotechnology
  • Concerns and Consequences
  • chapter 2: DNA, RNA, and Protein Synthesis
    • Structure of DNA
    • DNA Replication
    • Decoding Genetic Information: RNA and Protein
    • Translation
    • Regulation of mRNA Transcription in Bacteria
    • Regulation of mRNA Transcription in Eukaryotes
    • Protein Secretion Pathways
    chapter 3: Recombinant DNA Technology
    • Restriction Endonucleases
    • Plasmid Cloning Vectors
    • Creating and Screening a Library
    • Cloning DNA Sequences That Encode Eukaryotic Proteins
    • Vectors for Cloning Large Pieces of DNA
    • Genetic Transformation of Prokaryotes
    chapter 4: Chemical Synthesis, Amplification, and Sequencing of DNA
    • Chemical Synthesis of DNA
    • Polymerase Chain Reaction
    • DNA-Sequencing Techniques
    • Large-Scale DNA Sequencing
    chapter 5: Bioinformatics, Genomics, and Proteomics
    • Molecular Databases
    • Metagenomics
    • Functional Genomics
    • Proteomics
    chapter 6: Manipulation of Gene Expression in Prokaryotes
    • Gene Expression from Strong and Regulatable Promoters
    • Fusion Proteins
    • Translation Expression Vectors
    • Increasing Protein Stability
    • Overcoming Oxygen Limitation
    • Limiting Biofilm Formation
    • DNA Integration into the Host Chromosome
    • Increasing Secretion
    • Metabolic Load
    chapter 7: Heterologous Protein Production in Eukaryotic Cells
    • Posttranslational Modification of Eukaryotic Proteins
    • General Features of Eukaryotic Expression Systems
    • Fungus-Based Expression Systems
    • Baculovirus–Insect Cell Expression Systems
    • Mammalian Cell Expression Systems
    chapter 8: Directed Mutagenesis and Protein Engineering
    • Directed Mutagenesis Procedures
    • Protein Engineering
    PART II: Molecular Biotechnology of Microbial Systems
    chapter 9: Molecular Diagnostics
    • Immunological Diagnostic Procedures
    • Monoclonal Antibodies
    • Biofluorescent and Bioluminescent Systems
    • Nucleic Acid Diagnostic Systems
    • Molecular Diagnosis of Genetic Disease
    chapter 10: Protein Therapeutics
    • Pharmaceuticals
    • Enzymes
    • Lactic Acid Bacteria
    • Monoclonal Antibodies
    • Recombinant Antibodies
    chapter 11: Nucleic Acids as Therapeutic Agents
    • Antisense RNA
    • Ribozymes
    • Chimeric RNA–DNA Molecules
    • Aptamers
    • Interfering RNAs
    • Antibody Genes
    • Nucleic Acid Delivery
    chapter 12: Vaccines
    • Subunit Vaccines
    • Peptide Vaccines
    • Genetic Immunization: DNA Vaccines
    • Attenuated Vaccines
    • Vector Vaccines
    chapter 13: Synthesis of Commercial Products by Recombinant Microorganisms
    • Restriction Endonucleases
    • Lipase
    • Small Biological Molecules
    • Antibiotics
    • Biopolymers
    chapter 14: Bioremediation and Biomass Utilization
    • Microbial Degradation of Xenobiotics
    • Genetic Engineering of Biodegradative Pathways
    • Utilization of Starch and Sugars
    • Utilization of Cellulose
    • Hydrogen Production
    chapter 15: Plant Growth-Promoting Bacteria
    • Growth Promotion by Free-Living Bacteria
    • Biocontrol of Pathogens
    • Nitrogen Fixation
    • Hydrogenase
    • Nodulation
    • Phytoremediation
    chapter 16: Microbial Insecticides
    • Insecticidal Toxin of B. thuringiensis
    • Engineering of B. thuringiensis Toxin Genes
    • Baculoviruses as Biocontrol Agents
    chapter 17: Large-Scale Production of Proteins from Recombinant Microorganisms
    • Principles of Microbial Growth
    • Maximizing the Efficiency of the Fermentation Process
    • Bioreactors
    • Typical Large-Scale Fermentation Systems
    • Harvesting Microbial Cells
    • Disrupting Microbial Cells
    • Downstream Processing
    • Large-Scale Production of Plasmid DNA
    PART III: Molecular Biotechnology of Eukaryotic Systems
      chapter 18: Genetic Engineering of Plants: Methodology
      • Plant Transformation with the Ti Plasmid of A. tumefaciens
      • Ti Plasmid-Derived Vector Systems
      • Physical Methods of Transferring Genes to Plants
      • Chloroplast Engineering
      • Use of Reporter Genes in Transformed Plant Cells
      • Manipulation of Gene Expression in Plants
      • Production of Marker-Free Transgenic Plants
      chapter 19: Engineering Plants To Overcome Biotic and Abiotic Stress
      • Insect Resistance
      • Virus Resistance
      • Herbicide Resistance
      • Fungus and Bacterium Resistance
      • Oxidative Stress
      • Salt and Drought Stress
      • Fruit Ripening and Flower Wilting
      chapter 20: Engineering Plant Quality and Proteins
      • Modification of Plant Nutritional Content
      • Modification of Food Plant Taste and Appearance
      • Genetic Manipulation of Flower Pigmentation
      • Plants as Bioreactors
      • Edible Vaccines
      • Plant Yield
      • Phytoremediation
      chapter 21: Transgenic Animals
      • Transgenic Mice: Methodology
      • Transgenic Mice: Applications
      • Cloning Livestock by Nuclear Transfer
      • Transgenic Livestock
      • Transgenic Poultry
      • Transgenic Fish
      PART IV: Molecular Biotechnology and society
      chapter 22: Regulating the Use of Biotechnology
      • Regulating Recombinant DNA Technology
      • Deliberate Release of Genetically Modified Microorganisms
      • Regulating Food and Food Ingredients
      • Patenting Biotechnology
      chapter 23: Societal Issues in Biotechnology
      • Concerns about the Safety of Consuming Genetically Modified Foods
      • Concerns about the Impact of Genetically Modified Organisms on the Environment
      • Economic Issues
      • Amino Acids of Proteins and Their Designations
      • Glossary
      • Index
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